Link to home

Effects of Water Stress on Colonization of Poplar Stems and Excised Leaf Disks by Septoria musiva

April 1997 , Volume 87 , Number  4
Pages  381 - 388

D. L. Maxwell , E. L. Kruger , and G. R. Stanosz

First and third authors: Department of Plant Pathology; and second author: Department of Forestry, University of Wisconsin-Madison, 1630 Linden Drive, Madison 53706-1598

Go to article:
Accepted for publication 14 January 1997.

Septoria musiva causes leaf spot and canker diseases of trees in the genus Populus, and is one of the most damaging fungal pathogens of hybrid poplar in eastern North America. The effect of host water stress on Septoria canker development was studied in two separate greenhouse experiments. Hybrid poplar clones NM6, NC11396, and NE308 were stressed by withholding water until predawn water potential fell below −1.0 MPa. Stems were treated by removing a leaf and applying agar plugs that were either colonized by S. musiva (inoculated) or sterile (control) to the wound. Cankers on inoculated water-stressed trees were significantly larger than those on nonstressed trees. A leaf disk assay also was conducted three times with the NM6 and NE308 trees. We cut two disks from each of 120 stressed and 120 well-watered trees, placing them on water agar in 24-well tissue culture plates. A conidial suspension was applied to one disk in each pair and sterile water to the other. Inoculated disks from water-stressed trees developed less necrosis than those from well-watered trees. These results demonstrate that environmental influences on host condition must be considered in evaluating resistance of clones proposed for widespread culture of hybrid poplar.

Additional keywords: drought, Mycosphaerella populorum.

© 1997 The American Phytopathological Society