1Department of Entomology and Plant Pathology, Horticulture Research International, Kent ME19 6BJ, U.K.; 2Department of Biology, University of St. Andrews, Fife KY16 9ST, U.K.; 3Department of Virology, Institute of Microbiology, Beijing 100080, China; 4Department of Virus Research, John Innes Centre, Colney, Norwich NR4 7UH, U.K.
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Accepted 18 May 2001.
Expression of the Tomato yellow leaf curl virus-China (TYLCV-C) C2 protein and green fluorescent protein (GFP) fused to the C2 protein (C2-GFP) in Nicotiana benthamiana from a Potato virus X (PVX) vector induced necrotic ringspots on inoculated leaves as well as necrotic vein banding and severe necrosis on systemically infected leaves. The localization of GFP fluorescence in plant cells infected with PVX/C2-GFP and in insect cells transfected with Baculovirus expressing C2-GFP indicates that the TYLCV-C C2 protein is capable of shuttling GFP into plant and insect cell nuclei. Our data demonstrate that the TYLCV-C C2 protein may contribute to viral pathogenicity in planta and is nuclear localized.
putative transcriptional activator protein.
© 2001 The American Phytopathological Society