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Rhizobium sp. BR816 Produces a Complex Mixture of Known and Novel Lipochitooligosaccharide Molecules

May 2001 , Volume 14 , Number  5
Pages  678 - 684

Carla Snoeck , 1 Ellen Luyten , 1 Véréna Poinsot , 2 Arlette Savagnac , 2 Jos Vanderleyden , 1 and Jean-Claude Promé 2

1Centre of Microbial and Plant Genetics, Kasteelpark Arenberg 20, B-3001 Heverlee, Belgium; 2Laboratoire des Interactions Moléculaires et Réactivité Chimique et Photochimique, UMR 5623, Université Paul Sabatier, 118 Route de Narbonne, 31062 Toulouse Cedex, France

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Accepted 11 January 2001.

Rhizobial lipochitooligosaccharide (LCO) signal molecules induce various plant responses, leading to nodule development. We report here the LCO structures of the broad-host range strain Rhizobium sp. BR816. The LCOs produced are all pentamers, carrying common C18:1 or C18:0 fatty acyl chains, N-methylated and C-6 carbamoylated on the nonreducing terminal N-acetylglucosamine and sulfated on the reducing/terminal residue. A second acetyl group can be present on the penultimate N-acetylglu-cosamine from the nonreducing terminus. Two novel characteristics were observed: the reducing/terminal residue can be a glucosaminitol (open structure) and the degree of acetylation of this glucosaminitol or of the reducing residue can vary.

Additional keywords: host specificity, Nod factors, nodulation.

© 2001 The American Phytopathological Society