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Characterization of the ToxB Gene from Pyrenophora tritici-repentis

May 2001 , Volume 14 , Number  5
Pages  675 - 677

J. Patrick Martinez , 1 Sean A. Ottum , 1 Shaukat Ali , 2 Leonard J. Francl , 2 and Lynda M. Ciuffetti 1

1Department of Botany and Plant Pathology, Oregon State University, Corvallis 97331 U.S.A.; 2Department of Plant Pathology, North Dakota State University, Fargo 58105 U.S.A.

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Accepted 26 January 2001.

The ToxB gene was cloned and characterized from a race 5 isolate of Pyrenophora tritici-repentis from North Dakota. ToxB contains a 261-bp open reading frame that encodes a 23 amino acid putative signal peptide and a 64 amino acid host-selective toxin, Ptr ToxB. Analysis of Ptr ToxB from heterologous expression in Pichia pastoris confirms that ToxB encodes a host-selective toxin.

Additional keywords: pathogenicity, Ptr chlorosis toxin, tan spot, Triticum aestivum .

© 2001 The American Phytopathological Society