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Identification of an In Vitro Ribonucleoprotein Complex Between a Viroid RNA and a Phloem Protein from Cucumber Plants

July 2001 , Volume 14 , Number  7
Pages  910 - 913

Gustavo Gómez and Vicente Pallás

Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-CSIC, Av. de los Naranjos s/n, 46022 Valencia-Spain

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Accepted 19 March 2001.

We used the interaction of Hop stunt viroid (HSVd) and cucumber plants to investigate the involvement of phloem proteins in the systemic transport of RNA molecules. A ribonucleoprotein complex, stable even at high salt and temperature conditions, was detected in vitro between HSVd-RNA and the phloem exudate obtained from sectioned internodes from cucumber plants. The phloem protein 2 was recovered from this ribonucleoprotein complex and its RNA-binding properties as demonstrated by gel retardation analysis. The involvement of this protein in the movement of RNAs in cucumber is discussed.

© 2001 The American Phytopathological Society