Link to home

Construction of a Derivative of Agrobacterium tumefaciens C58 That Does Not Mutate to Tetracycline Resistance

January 2001 , Volume 14 , Number  1
Pages  98 - 103

Zhao-Qing Luo , 1 Thomas E. Clemente , 3 and Stephen K. Farrand 1 , 2

Departments of 1Crop Sciences; and 2Microbiology, University of Illinois at Urbana-Champaign, Urbana 61801, U.S.A.; 3Center for Biotechnology, University of Nebraska, Lincoln 68588, U.S.A.

Go to article:
Accepted 17 September 2000.

Agrobacterium tumefaciens C58 mutates to tetracycline resistance at high frequency, complicating the use of many broad-host-range cloning and binary vectors that code for resistance to this antibiotic as the selection marker. Such mutations are associated with a resistant gene unit, tetC58, that is present in the genome of this strain. By deleting the tetC58 locus, we constructed NTL4, a derivative of C58 that no longer mutates to tetracycline resistance. The deletion had no detectable effect on genetic or physiological traits of NTL4 or on the ability of this strain to transform plants.

Additional keywords: genetic engineering.

© 2001 The American Phytopathological Society