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Catharanthus roseus Genes Regulated Differentially by Mollicute Infections

February 2001 , Volume 14 , Number  2
Pages  225 - 233

Sandrine Jagoueix-Eveillard , Frank Tarendeau , Karine Guolter , Jean-Luc Danet , Joseph M. Bové , and Monique Garnier

Laboratoire de Biologie Cellulaire et Moléculaire, Institut de Biologie Végétale Moléculaire INRA & Université Victor Ségalen Bordeaux 2, BP 81, 33883 Villenave d'Ornon cedex, France

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Accepted 21 October 2000.

A differential display of mRNAs was used to isolate periwinkle cDNAs differentially expressed following infection with one of three mollicutes: Spiroplasma citri, Candidatus Phytoplasma aurantifolia, and stolbur phytoplasma. Twenty-four differentially expressed cDNAs were characterized by Northern blots and sequence analysis. Eight of them had homologies with genes in databanks coding for proteins involved in photosynthesis, sugar transport, response to stress, or pathways of phytosterol synthesis. The regulation of these genes in periwinkle plants infected by additional phloem-restricted bacteria showed that they were not specific to a given mollicute, but correlations with particular symptoms could be established. Expression of transketolase was down regulated following infection with a pathogenic strain of S. citri. No down regulation was observed for the nonphytopathogenic mutant GMT553, which is deficient for fructose utilization.

© 2001 The American Phytopathological Society