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Overexpression of Pti5 in Tomato Potentiates Pathogen-Induced Defense Gene Expression and Enhances Disease Resistance to Pseudomonas syringae pv. tomato

December 2001 , Volume 14 , Number  12
Pages  1,453 - 1,457

Ping He , 1 , 2 Randall F. Warren , 1 Tiehan Zhao , 1 Libo Shan , 1 , 2 Lihuang Zhu , 2 Xiaoyan Tang , 1 and Jian-Min Zhou 1

1Department of Plant Pathology, 4024 Throckmorton Plant Sciences Center, Kansas State University, Manhattan 66506, U.S.A.; 2Institute of Genetics, Chinese Academy of Sciences, Beijing 100101

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Accepted 30 July 2001.

The tomato Pti5 gene encodes a pathogen-inducible ethylene response element-binding protein-like transcription factor that interacts with the disease resistance gene product Pto. Overexpression of Pti5 or Pti5-VP16, a translational fusion with a constitutive transcriptional activation domain, in tomato enhanced resistance to Pseudomonas syringae pv. tomato. Constitutive expression of Pti5 or Pti5-VP16 did not affect the basal level of pathogenesis-related gene expression, but it accelerated pathogen-induced expression of GluB and Catalase. The results demonstrate a positive role of Pti5 in defense gene regulation and disease resistance and suggest that a pathogen-activated posttran-scriptional regulatory step is necessary for the pathogen induction of the defense gene expression.

Additional keywords: bacterial infection, GCC box, general resistance, transgenic plants.

© 2001 The American Phytopathological Society