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A Purine-Related Metabolite Negatively Regulates fixNOQP Expression in Sinorhizobium meliloti by Modulation of fixK Expression

April 2001 , Volume 14 , Number  4
Pages  572 - 576

Mario Soberón , 1 Claudia Morera , 1 Adam Kondorosi , 2 Oswaldo Lopez , 1 and Juan Miranda 1

1Instituto de Biotecnología, Departamento de Biología Molecular de Plantas, UNAM, Apdo postal 510-3, Cuernavaca, Morelos 62250, México; 2Institut des Sciences Vegetales, CNRS, Avenue de la Terrasse 1, Gif-Sur-Yvette 91198, France

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Accepted 12 December 2000.

5-aminoimidazole-4-carboxamide nucleotide (AICAR) is a negative effector of cytochrome terminal oxidase cbb3 production in Rhizobium etli. In this work, the effect of AICAriboside (AICAr), the precursor of AICAR on the expression of the Sinorhizobium meliloti fixNOQP operon encoding the symbiotic terminal oxidase cbb3, was analyzed. AICAr reduced the microaerobic induction levels of fixN-lacZ and fixT-lacZ gene fusions 18- and sevenfold respectively, and both genes were activated by the transcriptional activator FixK. A fixK-lacZ fusion presented 14-fold-reduced induction levels in microaerobic cell cultures in the presence of AICAr. AICAr also reduced three-fold the microaerobic expression levels of the nifA-lacZ fusion, whose expression as well as that of fixK is controlled by the two-component system FixL-FixJ. In contrast, AICAr had no effect on the expression levels of a hemA-lacZ fusion. These data suggest that AICAr prevents fixNOQP induction by the inhibition of fixK transcription.

© 2001 The American Phytopathological Society