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A New Peroxidase cDNA from White Clover: Its Characterization and Expression in Root Tissue Challenged with Homologous Rhizobia, Heterologous Rhizobia, or Pseudomonas syringae

September 1999 , Volume 12 , Number  9
Pages  825 - 828

Martin A. Crockard , Anthony J. Bjourson , and James E. Cooper

Department of Applied Plant Science, The Queen's University of Belfast, Newforge Lane, Belfast, BT9 5PX, Northern Ireland

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Accepted 11 May 1999.

Temporal reverse transcription-polymerase chain reaction (RT-PCR) expression analyses were performed on Trprx2, a new white clover peroxidase, with roots challenged with homologous rhizobia, heterologous rhizobia, and a pathogen, Pseudomonas syringae. Low levels of Trprx2 expression were evident in all rhizobial treatments but in P.syringae-treated clover background expression was dramatically reduced within 1 h and was undetectable in treatments inoculated for more than 3 h. Spraying 4 mM salicylic acid onto seedlings increased Trprx2 expression. These data suggest a defensive role for Trprx2 in white clover and indicate active defense suppression by the pathogen.

© 1999 The American Phytopathological Society