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Expression of the Ferrioxamine Receptor Gene of Erwinia amylovora CFBP 1430 During Pathogenesis

May 1999 , Volume 12 , Number  5
Pages  463 - 466

Alia Dellagi , Danièle Reis , Brigitte Vian , and Dominique Expert

Laboratoire de Pathologie Végétale, INRA / INA P-G, 16 rue Claude Bernard, F-75 231, Paris, France

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Accepted 22 January 1999.

Mutants of Erwinia amylovora CFBP 1430 lacking a functional high-affinity iron transport system mediated by desferrioxamine are impaired in their ability to initiate fire blight symptoms (A. Dellagi, M.-N. Brisset, J.-P. Paulin, and D. Expert. Mol. Plant-Microbe Interact. 11:734--742, 1998). In this study, a chromosomal transcriptional lacZ fusion was used to analyze the expression in planta of the E. amylovora ferrioxamine receptor gene foxR. LacZ activity produced by the strain harboring the fusion was highly induced in iron-restricted conditions and in inoculated apple leaf tissues. Microscopic observation revealed differential expression of this gene in relation to the localization and density of bacterial cells within the diseased tissue. Thus, the ability of bacterial cells to express their iron transport system in accordance with environmental conditions is likely important for disease evolution.

© 1999 The American Phytopathological Society