1Department of Plant Pathology, North Carolina State University, Raleigh 27695-7616, U.S.A.; 2Department of Plant Pathology, Iowa State University, Ames 50011, U.S.A.; 3Nematology, Wageningen Agricultural University, 6709 PD Wageningen, The Netherlands; 4Department of Plant Pathology, University of Georgia, Athens 30602-7274, U.S.A.
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Accepted 30 September 1998.
Polyclonal sera specific to β-1,4-endoglucanases (cellulases) synthesized in the subventral esophageal gland cells of the soybean cyst nematode, Heterodera glycines, were used to provide the first identification of a nematode esophageal gland protein that is secreted into host plant tissue. Sera generated to proteins encoded by Hg-eng-1 and Hg-eng-2 (endoglucanases) did not cross-react with soybean root proteins on Western blots (immunoblots) or in immunofluorescence microscopy of noninoculated (control) soybean root sections. In cross sections of soybean roots at 24 h after inoculation of roots with second-stage juveniles of H. glycines, HG-ENG-1 was localized within the nematode's subventral gland cells and was not detected in root tissue. HG-ENG-2 was localized within the subventral gland cells and was secreted from the juvenile's cortical tissue at 24 h after inoculation of roots with second-stage juveniles of H. glycines. HG-ENG-2 was localized along the juvenile's migratory path through the root cortex.
© 1999 The American Phytopathological Society