Virology Department, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, Scotland
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Accepted 5 May 1999.
Replicase-mediated resistance to cucumber mosaic virus (CMV) affects CMV replication and restricts CMV movement. Confocal laser scanning microscopic analysis of resistant plants inoculated with either CMV or potato virus X, expressing the CMV movement protein (MP) fused to the green fluorescent protein (GFP), showed that the CMV MP was not inhibited from either plasmodesmal association or trafficking in the CMV-resistant plants. CMV expressing free GFP was able to move to adjacent cells, demonstrating that replicase-mediated resistance did not directly block the trafficking of CMV RNA.
© 1999 The American Phytopathological Society