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Agrobacterium tumefaciens Transformation of the Radiation Hypersensitive Arabidopsis thaliana Mutants uvh1 and rad5

November 1998 , Volume 11 , Number  11
Pages  1,136 - 1,141

Jaesung Nam , 1 Kirankumar S. Mysore , 2 and Stanton B. Gelvin 1 , 2

1Department of Biological Sciences, 2Purdue Genetics Program, Purdue University, West Lafayette, IN 47907-1392 U.S.A.

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Accepted 10 July 1998.

The Arabidopsis thaliana mutants uvh1 and rad5, originally identified as radiation hypersensitive, were reported to be deficient in T-DNA integration based on the relative efficiencies of stable transformation and T-DNA transfer. We reassessed these mutants for susceptibility to transformation by Agrobacterium tumefaciens. The mutant rad5 showed a significant reduction in the efficiency of transient as well as stable transformation, compared with its wild-type progenitor. These data indicate that rad5 is blocked at a step in the transformation process prior to T-DNA integration. We additionally found, using both an in vitro root inoculation and an in vivo flower bolt inoculation assay, that the mutant uvh1 is as susceptible to A. tumefaciens-mediated transformation as is its wild-type progenitor, C10.

Additional keywords: crown gall tumors.

© 1998 The American Phytopathological Society