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Mutagenesis of Putative Acylation Sites Alters Function, Localization, and Accumulation of a Giα Subunit of the Chestnut Blight Fungus Cryphonectria parasitica

November 1998 , Volume 11 , Number  11
Pages  1,130 - 1,135

Shaojian Gao and Donald L. Nuss

Center for Agricultural Biotechnology, University of Maryland Biotechnology Institute, University of Maryland, College Park 20742-3351 U.S.A.

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Accepted 30 June 1998.

Targeted disruption of cpg-1, a gene encoding the G protein Giα subunit, CPG-1, in the chestnut blight fungus, Cryphonectria parasitica, results in reduced mycelial growth, reduced orange pigmentation, loss of virulence, loss of asexual sporulation, and female infertility. We report the development of a complementation system for cpg-1 null mutants and its use to evaluate the in vivo consequences of mutating conserved putative CPG-1 myristoylation (G2) and palmitoylation (C3) sites. Independent mutations of the two putative acylation sites differentially altered complex fungal biological processes, including virulence, and modified CPG-1 membrane association. Results of combined Northern (RNA) and Western (immunoblot) analysis also indicated a role for lipid modification in post-transcriptional regulation of CPG-1 accumulation.

Additional keywords: fungal virulence.

© 1998 The American Phytopathological Society