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Use of Green Fluorescent Protein to Detect Expression of nif Genes of Azoarcus sp. BH72, a Grass-Associated Diazotroph, on Rice Roots

January 1998 , Volume 11 , Number  1
Pages  71 - 75

Tanja Egener , Thomas Hurek , and Barbara Reinhold-Hurek

Max-Planck-Institut für terrestrische Mikrobiologie, Arbeitsgruppe Symbioseforschung, Karl-von-Frisch-Str., D-35043 Marburg, Germany

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Accepted 24 September 1997.

A gfp (green fluorescent protein) cassette for transcriptional fusions has been developed to study gene expression in Azoarcus sp. BH72 in association with plant roots. The bacteria expressed nitrogenase genes (nifHDK) in the rhi-zosphere, on root tips, and in epidermal cells of rice seedlings. Green fluorescent protein fusions also visualized promoter activity of single cells in soil.

Additional keywords: nitrogen fixation.

© 1998 The American Phytopathological Society