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Differential Display Analysis of the Early Compatible Interaction Between Soybean and the Soybean Cyst Nematode

December 1998 , Volume 11 , Number  12
Pages  1,258 - 1,263

Dieter Hermsmeier , Mitra Mazarei , and Thomas J. Baum

Department of Plant Pathology, Iowa State University, 351 Bessey Hall, Ames 50011, U.S.A.

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Accepted 18 August 1998

The marked cellular changes during feeding site formation of the soybean cyst nematode (Heterodera glycines) indicate major changes in soybean gene expression. We used differential display of mRNA to detect host gene expression changes during the early compatible interaction between soybean and H. glycines. Fifteen cDNA clones corresponding to mRNAs with different abundances in H. glycines-infected versus uninfected roots were identified. Differential display results indicated that abundances of five mRNAs increased in infected roots, whereas abundances of 10 mRNAs decreased. Transcripts for nine of these 15 cDNAs could be detected on RNA blots, and their hybridization signals confirmed the differential display results for eight of these nine cDNAs. Sequence analyses identified five cDNAs with decreased mRNA levels in infected roots as corresponding to two putative aldolase genes, a transcription-factor TFIIA homologue, the soybean small GTP-binding protein gene sra1, and the soybean auxin down-regulated gene ADR12. RNA blot analyses of other auxin down-regulated genes revealed a decrease in their mRNA abundances in H. glycines-infected roots as well.

Additional keywords: syncytium.

© 1998 The American Phytopathological Society