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Tobacco Mosaic Virus Helicase Domain Induces Necrosis in N Gene-Carrying Tobacco in the Absence of Virus Replication

December 1998 , Volume 11 , Number  12
Pages  1,242 - 1,246

Truus E. M. Abbink , Pernilla A. Tjernberg , John F. Bol , and Huub J. M. Linthorst

Institute of Molecular Plant Sciences, Gorlaeus Laboratories, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands

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Accepted 2 September 1998.

Tobacco mosaic virus (TMV) elicits a hypersensitive response (HR) in tobacco plants that carry the N gene. To identify the elicitor of this HR, Agrobacteriumn tumefaciens was used as a vector for the transient expression of TMV replicase proteins, movement protein, and coat protein in NN and nn tobacco. Transient expression of the 126K protein and fragments thereof containing the helicase motifs induced necrosis and systemic expression of the pathogenesis-related PR-1a gene in NN plants but not in nnplants. The results confirm previous evidence that the TMV helicase sequence is the elicitor of the HR (H. S. Padgett, Y. Watanabe, and R. N. Beachy, Mol. Plant-Microbe Interact. 10:709-715, 1997) and demonstrate that this helicase sequence acts as an elicitor of HR in the absence of other viral proteins or RNA replication.

© 1998 The American Phytopathological Society