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Encapsidation of Potyviral RNA in Various Forms of Transgene Coat Protein Is Not Correlated with Resistance in Transgenic Plants

November 1997 , Volume 10 , Number  8
Pages  1,023 - 1,027

John Hammond and Margaret M. Dienelt

United States Department of Agriculture, Agricultural Research Service, United States National Arboretum, Floral and Nursery Plants Research Unit, Beltsville, MD 20705, U.S.A.

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Accepted 11 August 1997.

Transgenic plants expressing either bean yellow mosaic potyvirus or chimeric potyvirus coat protein (CP) were inoculated with various potyviruses. Antigen-coated plate, indirect enzyme-linked immunosorbent assay and immunoelectron microscopy of virus purified from transgenic plants showed that progeny virions contained from <1% to as much as 25% transgenic CP. Different levels of transcapsidation may reflect the extent of compatibility between transgene CP and the viral CP.

Additional keywords: aphid transmissibility, phenotypic mixing.

The American Phytopathological Society, 1997