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Alfalfa Mosaic Virus Movement Protein Induces Tubules in Plant Protoplasts

November 1997 , Volume 10 , Number  8
Pages  1,010 - 1,014

Huanquan Zheng , Guiling Wang , and Lee Zhang

The Laboratory of Plant Virology and Biotechnology, Institute of Molecular Agrobiology, 59A, The Fleming, 1 Singapore Science Park Drive, Singapore 118240

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Accepted 14 July 1997.

The alfalfa mosaic virus (AlMV) movement protein (MP) was fused with the green fluorescence protein (GFP). The MP-GFP fusion was transiently expressed in protoplasts prepared from Nicotiana tabacum, resulting in the production of long, extending, tubular structures protruding from the protoplast surface. Deletions of MP amino acids 1 to 77, 84 to 142, or 226 to 300 all affected tubule formation. Hence, the AlMV MP is solely required for the induction of the tubular structures, and the results suggest that AlMV may move from cell to cell via tubules.

© 1997 The American Phytopathological Society