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High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes II. Pi-ta² and Pi-ta and a Consideration of Their Origin

¹National Institute of Agrobiological Resources (NIAR), Kan'non-dai, Tsukuba, Ibaraki 305, Japan; ²Plant Breeding and Acclimatization Institute, Radzikow, Blonie, Poland; ³Institute of Plant Biotechnology IBARAKI, Ago, Iwama, Ibaraki 309-02, Japan; ⁴Ibaraki University, Faculty of Biological Sciences, Ami, Ibaraki 300-03, Japan; ⁵National Agricultural Research Center, Kan'non-dai, Tsukuba, Ibaraki 305, Japan; ⁶PRESTO Research Development Corporation of Japan, Honcho, Kawaguchi, Saitama 332, Japan

The indica-derived rice blast resistance genes Pi-ta² and Pi-ta, introgressed into a japonica background, were mapped on the middle of chromosome 12 with restriction fragment length polymorphism (RFLP)/random amplified polymorphic DNA (RAPD) markers and several nearly isogenic lines (NILs). Pi-ta² cosegregated with XNpb 289 and 196, and was flanked by closely linked markers at 0.3 (ta²-1) and 0.7 cM (XNpb 088, 319/079) on the left (up) side, and at 0.3 (RUBss) and 0.7 cM (XNpb316) on the right (down) side of the map, in F₂ analyses of Pi No. 4 × japonica crosses, with 160 recessive homozygotic individuals corresponding to 320 chromosomes. CHEF (contour-clamped hexagonal electric field) Southern blotting suggested that XNpb088 and XNpb239-1, both very close to Pi-ta², were spanned by a 170-kb band, suggesting about 500 to 750 kb/cM as the physical/genetic distances ratio in the crosses of Pi No. 4 × japonica, which was rather low in recombination ratio, in this region. Pi-ta was mapped to the position overlapping Pi-ta² by graphical genotype analysis of an NIL with a very narrow introgressed region, Shimokita. This result suggested that these functionally related genes are allelic or at least very closely located, and that they may be derived from a common ancestral gene. However, RFLP analyses of the NILs of these two genes indicated that they are derived from rather different genomes, refuting the report that NILs of Pi-ta share a common donor with those of Pi-ta². This indicates that the two genes differentiated at a rather ancient time. Two other pedigrees, describing spontaneous recent origins of Pi-ta in japonica, were also refuted and a single indica origin of the gene was indicated.

Additional keywords: Magnaporthe grisea, Oryza sativa.