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Defense Response in Slash Pine: Chitosan Treatment Alters the Abundance of Specific mRNAs

¹USDA Forest Service, Southern Institute of Forest Genetics, Harrison Experimental Forest, 23332 Hwy 67, Saucier MS 39574 U.S.A.; ²School of Forest Resources and Conservation, and Plant Molecular and Cellular Biology Program, Box 110410, University of Florida, Gainesville FL 32611 U.S.A.

We used differential display to identify chitosan responsive cDNAs in slash pine cell cultures. Two clones that showed increased mRNA abundance had sequence similarity to genes with roles in major plant defense responses, clone 18 to cinnamic acid 4-hydroxylase, and clone 30 to chitinase.

Additional keywords: cytochrome P450, elicitation, Pinus elliottii.