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The Vicia faba Leghemoglobin Gene VfLb29 Is Induced in Root Nodules and in Roots Colonized by the Arbuscular Mycorrhizal Fungus Glomus fasciculatum

January 1997 , Volume 10 , Number  1
Pages  124 - 131

Martin Frühling , 1 Hélène Roussel , 2 Vivienne Gianinazzi-Pearson , 2 Alfred Pühler , 1 and Andreas M. Perlick 1

1Universität Bielefeld, Lehrstuhl für Genetik, Postfach 100131, D-33501 Bielefeld, Germany; 2Station de Génétique et d' Amélioration des Plantes, INRA, BV 1540, F-21034 Dijon cédex, France

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Accepted 30 October 1996.

To investigate similarities between symbiotic interactions of broad bean (Vicia faba) with rhizobia and mycorrhizal fungi, plant gene expression induced by both microsymbionts was compared. We demonstrated the exclusive expression of 19 broad bean genes, including VfENOD2, VfENOD5, VfENOD12 and three different leghemoglobin genes, in root nodules. In contrast, the leghemoglobin gene VfLb29 was found to be induced not only in root nodules, but also in broad bean roots colonized by the mycorrhizal fungus Glomus fasciculatum. In uninfected roots, none of the 20 nodulin transcripts investigated was detectable. VfLb29 has an unusually low sequence homology with all other broad bean leghemoglobins as well as with leghemoglobins from other legumes. It can be regarded as a novel kind of leghemoglobin gene not described until now and the induction of which is common to symbiotic interactions of broad bean with both Rhizobium and a mycorrhizal fungus.

© 1997 The American Phytopathological Society