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High Resolution Genetic and Physical Mapping of Molecular Markers Linked to the Phytophthora Resistance Gene Rps1-k in Soybean

December 1997 , Volume 10 , Number  9
Pages  1,035 - 1,044

Takao Kasuga , 1 Shanmukhaswami S. Salimath , 1 Jinrui Shi , 1 Mark Gijzen , 2 Richard I. Buzzell , 3 and Madan K. Bhattacharyya 1

1The Samuel Roberts Noble Foundation, P.O. Box 2180, Ardmore, OK 73402, U.S.A.; 2Agriculture Canada, 1391 Sandford Street, London, Ontario, Canada N5V 4T3; 3Agriculture Canada, Harrow, Ontario, Can-ada N6G 2V4

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Accepted 18 June 1997.

The resistance of soybean to Phytophthora root and stem rot caused by Phytophthora sojae is conferred by a series of single-dominant Rps genes. We have applied random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) analyses to isolate molecular markers linked to Rps1-k. Five RAPD markers were identified and mapped closely to one side of Rps1-k. AFLP analysis was carried out with near isogenic lines and bulks obtained from F3 families. Twenty-seven markers were identified. Nineteen of these were specific to the resistant parent. Five AFLP markers were amplified from the susceptible parent. One of these markers, TC1, mapped at 0.07 centimorgans (cM) from the Rps1 locus. Three AFLP markers were co-dominant, and one of these, CG1, mapped at a distance of 0.06 cM from the Rps1 locus on the opposite side of the rest of the markers. Two RAPD, 17 AFLP, and 14 restriction fragment length polymorphism (RFLP) markers originating from duplicated sequences were mapped within a 3-cM map interval. These results suggest that Rps1-k is located at the end of an introgressed region. Physical mapping data indicate that the Rps1-k-flanking markers CG1 and TC1 may be located within a 125-kb chromosomal fragment.

© 1997 The American Phytopathological Society