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Ceratocystis fimbriata transmission by vegetative propagation in Eucalyptus urograndis

Jéssica Karine Burim Cardoso: São Paulo State University

<div>This work aimed to verify the possibility of disseminating <em>Ceratocystis fimbriata</em> by vegetative propagation, and whether the clonal seedlings constitute the inoculum source of this pathogen. For this were chosen parents of the clone SP-519 (<em>Eucalyptus urograndis</em>), susceptible to the pathogen, planted at a location with a history of occurrence of the pathogen, to form of a clonal garden. From these collected buddings, microcuttings were removed and submitted to the process of rooting and acclimation, obtaining 96 seedlings, which were transplanted to a receptacle containing sterilized washed sand, with ferti-irrigation. Three of the 96 plants were observed to die at days 13, 93 and 139, respectively, after transplantation, indicating 3.13% mortality. Each plant was isolated for morphological and molecular characterization of the pathogen, which demonstrated the presence of <em>C. fimbriata</em>. This first observation evidenced that the disease can be transmitted by vegetative propagation. Seedlings are being obtained from these parents. Isolations are being carried out while specific primers are being designed to monitor healthiness by PCR. Seedling producers must be warned of the risk and a healthiness test must be recommended for the formation of parent plants for nursery cultivators.</div>