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Characterization of antimicrobial-producing beneficial bacteria from citrus rhizosphere
Nadia Riera: University of Florida; Yunzeng Zhang: CREC, University of Florida; Utpal Handique: University of Florida; Nian Wang: University of Florida
<div>The microbiomes associated with crop plants have a strong impact on plant health and productivity. Here, we present our work on the isolation and characterization of putative beneficial bacteria from healthy citrus rhizosphere and test whether they can be used to control citrus diseases caused by <i>Candidatus</i> Liberibacter asiaticus (Las) and Phytophthora. Six isolates were tested for antibacterial activity against three bacteria closely related to Las: <i>Agrobacterium tumefaciens</i>, <i>Sinorhizobium meliloti</i> and <i>Liberibacter crescens</i> since Las has not been cultured. We also test the antifungal activity. Four isolates with antagonistic activity against the citrus root pathogen <i>Phythopthora</i> <i>nicitianae</i> were identified to belong to <i>Stenotrophomonas</i>, Bacilli and <a href="https://www.google.com/search?q=to+Stenotrophomonas,+Bacilli+and+Burkholderiaceae+families&spell=1&sa=X&ved=0ahUKEwiyhKTrkdTSAhWD2SYKHZJADPUQBQgZKAA">Burkholderiaceae families</a>;. We sequenced the genomes of<i> Pseudomonas geniculata</i> strain 95 and <i>Bacillus pumilus</i> strain 104 to explore genes associated with beneficial traits. We will discuss how the identified antimicrobial-producing bacteria contribute to disease control.</div>

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