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Expanding the scope of a single pathogen survey to solve the mystery of the ailing red raspberries
Jerry Weiland: USDA ARS; Chris Benedict: WSU; Inga Zasada: USDA ARS; Bryan Beck: USDA ARS; Anne Davis: USDA ARS; Amy Peetz: USDA ARS HCRU; Kim Graham: USDA ARS; Robert Martin: USDA ARS; Jeremiah Dung: Oregon State University; Andres Reyes Gaige: Oregon State University; Lindsey Thiessen: North Carolina State University
<div>Washington state produces almost 60% of the processed raspberries ($79 M value) in the U.S. Production is severely limited by Raspberry Bushy Dwarf Virus (RBDV) and the soilborne pathogens <i>Phytophthora rubi</i> and <i>Pratylenchus penetrans</i>. However, in 2012, growers began noticing plants with unusual symptoms that were not attributed to any of these pathogens. Starting in late summer, individual primocanes or entire plants would suddenly wilt and die. Adjacent plants were often chlorotic and stunted. Early diagnostic work indicated that <i>Verticillium dahliae</i> was the culprit at three locations, but analyses of soil populations often produced conflicting results depending on the lab and detection method. Growers became concerned that this pathogen was a previously undiagnosed problem for the industry. Therefore, a survey was conducted to determine if <i>V. dahliae</i> was common in production fields, to determine if <i>V. dahliae </i>was associated with disease, and to compare the sensitivity of two detection methods (selective media versus qPCR) for quantifying <i>V. dahliae </i>in the soil. The presence of <i>P. rubi</i>, <i>P. penetrans</i>, and RBDV was also assessed. In 2013 to 2014, primocanes, roots, and soil were collected from 51 disease sites and 20 healthy sites within 24 production fields. Results show that <i>V. dahliae</i> is common and that qPCR is more sensitive than plating for detection. But, the main question remains, which pathogen(s) are causing the disease? Come find out!</div>

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