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Strain specificity of helper components encoded by Potato virus Y
Shaonpius Mondal: Cornell University; Gary Stewart: Cornell University
<div>In recent years, the recombinant strains of <i>Potato virus Y</i> (PVY<sup>NTN</sup> and PVY<sup>N:O</sup>) have steadily replaced their nonrecombinant parent, PVY<sup>O</sup>, in the U.S. potato crop. Previous studies showed a transmission bias for recombinant strains when acquired sequentially with PVY<sup>O</sup>, regardless of acquisition order. To understand this bias, the nonstructural helper component (HC) and infectious virus particles were separated, mixed in various combinations, and fed to green peach aphids using Parafilm® sachets. Following a 20 min acquisition access period, groups of 10-12 aphids were transferred to potato seedlings for a 24 h inoculation access period. Treatments included all possible combinations of HC and purified virus (PV) from PVY<sup>O</sup>, PVY<sup>N:O</sup>, and PVY<sup>NTN</sup>. Plants were assayed 21-28 days post inoculation by TAS-ELISA. When heterologous or homologous combinations of PV and HC of PVY<sup>N:O</sup> and PVY<sup>NTN </sup>were mixed and fed immediately to aphids, transmission from any treatment was <20%, except the PVY<sup>NTN</sup> PV - PVY<sup>N:O </sup>HC combination (>60%). Following a 3-4 h incubation of PV with HC at 4°C, transmission efficiency of the all combinations increased to >70% except the PVY<sup>N:O</sup> PV - PVY<sup>NTN </sup>HC combination, which remained <20%. These data indicate a strain specific function of HC, and suggest that formation of a HC-PV protein complex prior to aphid acquisition facilitates transmission. Ongoing research is determining transmission of HC-PV combinations of PVY<sup>O</sup>-PVY<sup>N:O </sup>and PVY<sup>O</sup>-PVY<sup>NTN</sup>.</div>

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