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Marker development for Puccinia striiformis f. sp. tritici
Xiaoping Hu: Northwest A&F University, China
<div>Strip rust, caused by <i>Puccinia striiformis</i><i> </i>Westend. f. sp. <i>tritici</i> Eriks. (<i>Pst</i>), results in severe economic losses for wheat growers in many countries. The current <i>Pst</i> race identification mainly depends on the virulence phenotype of the pathogen on a set of wheat differential genotypes. Usually, the approach needs increasing enough urediniospores and testing a set of differential genotypes in a conditioned greenhouse. Such testing can be limited by space and temperature conditions, and is also time-consuming and labor-intensive. Usually, one person can only identify ca. 400 <i>Pst</i> samples per year. <p>In 1990s, the moderately repetitive sequences from <i>Pst</i> were developed to identify 96 genotypes among 160 isolates in the Chinese <i>Pst</i> population. In 2000s, RAPD-SCAR markers of CYR17, CYR29, CRY31, CYR32, CYR33 and V26 were developed to identify races from large scale field samples. In 2009, we identified 20 new polymorphic microsatellite loci derived from the expressed sequence tag (EST) data of Pst. Since 2015, we developed 5741 SNPs using 15 RNA-seq data set of <i>Pst</i> race CYR32 compared to PST78, among which 67 SNPs associated with 13 SP-SNP (secreted protein SNP) were identified. Now, we are developing SNPs related to virulence variation of<i> Pst</i> based on 600 whole-genome re-sequence data set. <p>In summary, these RAPD-SCARs, EST-derived SSRs, and SNPs will be particularly valuable for virulence-related gene discovery as well as for studies on population evolution, population diversity, and dispersal route of <i>Pst</i>.</div>

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