|Differential proteomic profiles of sugarbeet resistance genes Rz1 and Rz2 during interactions with Beet necrotic yellow vein virus|
K. M. WEBB (1), W. M. Wintermantel (2), L. Hldaky (2), P. Covey (1), C. J. Broccardo (3), J. E. Prenni (3). (1) USDA ARS NPA SBRU, Fort Collins, CO, U.S.A.; (2) USDA ARS CIPRU, Salinas, CA, U.S.A.; (3) Colorado State University, Fort Collins, CO, U.S.A.
Rhizomania, caused by<i> Beet necrotic yellow vein virus </i>(BNYVV), is an economically important disease affecting sugarbeet production by reducing root quality and sugar yield. A number of resistance genes have been identified for control; however, new pathotypes that overcome resistance have emerged. Understanding sugarbeet proteins involved in resistant and susceptible interactions with BNYVV may lead to novel management strategies. Three near isogenic sugarbeet lines; one susceptible to all forms of BNYVV and resistant lines separately, carrying the <i>Rz1 </i>and <i>Rz2</i> resistance genes, were infected with two sources of BNYVV; standard BNYVV A-type, and the <i>Rz1 </i>resistance-breaking BNYVV-IV. Seedlings were grown in a growth chamber at 24oC with 16 hour days. At 3 weeks post-germination plants were harvested and foliar and root portions of the plant were separated and pooled. Root samples from each pot were tested by ELISA to confirm BNYVV infection and total protein was extracted from lyophilized leaf tissue. Protein extracts were processed via LC-MS/MS and subsequently separated on a reverse phase nanospray column. Peptide spectra were examined for protein identity using a Uniprot <i>Amaranthaceae</i> database. Differential protein expression was evaluated and several proteins were found to be more highly expressed during comparisons between compatible and incompatible interactions suggesting different biological pathways that may lead to novel management and breeding targets.