Comparative genomics of Ralstonia solanacearum strain P781 that infects Mandevilla and Dipladenia plants
A. M. BOCSANCZY (1), J. M. F. Yuen (2), A. J. Palmateer (3), D. J. Norman (1). (1) Univ of Florida Mid-Florida Research and Education Center, Apopka, FL, U.S.A.; (2) University of Florida Mid-Florida Research and Education Center, Apopka, FL, U.S.A.; (3) University of Florida Tropical research and Education Center, Homestead, FL, U.
<i>Ralstonia solanacearum </i>causes Bacterial Wilt in more than 200 species of plants. Recently, we isolated <i>R. solanacearum</i> strains from production of Mandevilla and Dipladenia in Florida. Many of the cultivars infected are now crosses of both genera, referred as ‘Sundavilla’. We confirmed the strains as biovar 3. A limited host range test was performed on tomato, geranium, <i>Dipladenia sanderi</i>, and <i>Mandevilla boliviensis</i> with biovar 3 control strain GMI1000 along with three isolated strains. GMI1000 was pathogenic and highly virulent on tomato plants, while weakly virulent on the other hosts. The other strains were highly virulent on all hosts. Host specificity of bacterial pathogens has been linked to type III effectors. We seek to identify type III effectors that may restrict the host range of GMI1000 to solanaceous plants. The genome of strain P781 selected from the group of three strains was sequenced using the Illumina paired-end technology. More than 9 million reads were assembled in 262 contigs. Preliminary genome annotation and assembly in gapped molecules was done. Phylogenetic closeness to GMI1000 was confirmed by comparison of the endoglucanase gene sequence with <i>R. solanacearum</i> genome sequences available. Currently, the genome sequence is in process of manual curation and identification of type III effectors. Comparative genomics of the annotated sequence of P781 with GMI1000 genome is expected to reveal potential host specificity factors.
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