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Root-knot nematode <i>M. enterolobii</i> has a high reproductive rate and a wide host range, and is currently spreading in many agricultural crops around the world. From 2007 to 2010, we surveyed the incidence of root-knot nematodes in Hainan Province of China on three important Chinese medicinal crops: noni (<i>Morinda citrifolia</i>), Chinese eaglewood (<i>Aquilaria agallocha</i>) and clove (<i>Syzygium aromaticum</i>). Samples were collected from roots of each crop and nematode species were identified by morphology, isoenzyme technology and mtDNA marker. Measurements of the second-stage juveniles and morphological characteristics of the female perineal patterns coincided with the description of <i>M. enterolobii</i>. The perineal patterns were variable, with moderately high to high dorsal arch and with no lateral lines. Isoenzyme experiments showed a strong malatedehydrogenase (Mdh) band (N1a) and a weaker esterase (Est) band (VS1-S1). PCR amplification of the mtDNA between <i>COII</i> and <i>lrRNA</i> gene was accomplished with primers #C2F3 (5’-GGTCAATGTTCAGAAATTTGTGG-3’) and #1108 (5’-TACCTTTGAC CAATCACGCT-3’). A product of 0.7 kb was obtained and the fragments could not be digested with restriction enzyme <i>Hinf</i>I<i>.</i> All these results confirmed the presence of root-knot nematode. To our knowledge, this is the first record of <i>M. enterolobii</i> parasitizing noni, Chinese eaglewood and clove in Hainan, China.<p><p>Keywords: Nematode, Tropical-Subtropical Crops