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First report of Meloidogyne enterolobii on Noni, Chinese Eaglewood, and Clove in China.
W. H. Fang (1), F. M. Ying (2), C. M. CAI (2). (1) Institute of Environment and Plant Protection, Haikou, Peoples Republic of China; (2) Hainan Academy of Agricultural Sciences, Haikou, Peoples Republic of China

Root-knot nematode <i>M. enterolobii</i> has a high reproductive rate and a wide host range, and is currently spreading in many agricultural crops around the world. From 2007 to 2010, we surveyed the incidence of root-knot nematodes in Hainan Province of China on three important Chinese medicinal crops: noni (<i>Morinda citrifolia</i>), Chinese eaglewood (<i>Aquilaria agallocha</i>) and clove (<i>Syzygium aromaticum</i>). Samples were collected from roots of each crop and nematode species were identified by morphology, isoenzyme technology and mtDNA marker. Measurements of the second-stage juveniles and morphological characteristics of the female perineal patterns coincided with the description of <i>M. enterolobii</i>. The perineal patterns were variable, with moderately high to high dorsal arch and with no lateral lines. Isoenzyme experiments showed a strong malatedehydrogenase (Mdh) band (N1a) and a weaker esterase (Est) band (VS1-S1). PCR amplification of the mtDNA between <i>COII</i> and <i>lrRNA</i> gene was accomplished with primers #C2F3 (5’-GGTCAATGTTCAGAAATTTGTGG-3’) and #1108 (5’-TACCTTTGAC CAATCACGCT-3’). A product of 0.7 kb was obtained and the fragments could not be digested with restriction enzyme <i>Hinf</i>I<i>.</i> All these results confirmed the presence of root-knot nematode. To our knowledge, this is the first record of <i>M. enterolobii</i> parasitizing noni, Chinese eaglewood and clove in Hainan, China.<p><p>Keywords: Nematode, Tropical-Subtropical Crops

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