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Our expanding SolaR80 system: Toward comprehensive survey of the solanaceae R-gene space.
J. BRADEEN (1), E. Quirin (1), H. Mann (1), A. Traini (2), M. L. Chiusano (2), D. Carputo (2). (1) University of Minnesota, St. Paul, MN, U.S.A.; (2) University of Naples Federico II, Portici, Italy

Most plant disease resistance (R) genes encode a conserved nucleotide binding site (NBS) and a variable leucine rich repeat (LRR) domain. NBS R gene fragments (Resistance Gene Analogs; RGAs) have been amplified from the genomes of many plant species via PCR. We amplified 97 RGAs from the wild potato <i>Solanum bulbocastanum</i> and combined these, RGAs from eight other <i>Solanum</i> species and sequences of previously cloned Solanaceae R genes into a single meta-analysis. Based on an 80% DNA sequence homology threshold, nearly 800 NBS sequences were assigned to 56 diversity bins that we call “SolaR80” (SOLAnaceae R gene) groups. We demonstrated that SolaR80 groups reflect both DNA homology and evolutionary relationships among the R gene sequences. Distribution of SolaR80 groups across <i>Solanum</i> species was arrayed adjacent to a neighbor joining tree illustrating relationships among the SolaR80 groups. The result is the SolaR80 System. This system provides a simple visual means of exploring cross-species distribution of R gene families, reveals patterns of allelic diversification, and facilitates comparative genomics approaches by establishing a common terminology. Importantly, this system is also amendable and expandable. Current research efforts include genome-wide analysis of tomato and potato genome sequences to identify NBS-LRR genes for integration into the SolaR80 System and generation of RGA resources for strategic <i>Solanum</i> species using next generation sequencing.<p><p>Keywords: NA, Vegetables, Tomato

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