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Distribution and detection of Botrytis species of blackberry and strawberry in the Southeast United States.
X. LI (1), D. Fernandez-Ortuno (1), A. Grabke (1), G. Schnabel (1). (1) Clemson University, Clemson, SC, U.S.A.

<i>Botrytis</i> spp. cause blossom blight and fruit rot on many crops, including strawberry and blackberry. In 2011, 400 isolates from blackberry and strawberry fields were collected from North and South Carolina and single-spore colonies were obtained and characterized. Two distinct species, <i>B. cinerea</i> Pers. and another newly described <i>Botrytis</i> species – <i>Botrytis caroliniana</i> X.P. Li & G. Schnabel, were identified based on examination of the coding G3PDH, HSP60, RPB2 and NEP1 genes and based on morphological characters. A PCR method was developed to distinguish <i>B. cinerea</i> from <i>B. caroliniana</i> but cultural characteristics can also be used for rapid distinction. Without exception, <i>B. cinerea</i> isolates sporulated on potato dextrose agar and kings medium B, while <i>B. caroliniana</i> isolates did not. The new species is pathogenic on blackberry, strawberry, broad beans and tomatoes, which is the second <i>Botrytis</i> species that has a broad host range besides <i>Botrytis cinerea</i>. Isolates from both species were subjected to cyprodinil, fenhexamid, fludioxonil, pyraclostrobin, boscalid, thiophanate-methyl and iprodione sensitivity evaluations using germination assays and several different fungicide resistance phenotypes were discovered.<p><p>Keywords: Fungus, Fruits-Nuts, Cane Berry

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