Link to home

Cannot retrieve the URL specified in the Content Link property. For more assistance, contact your site administrator.

TaqMan qPCR detection of three berry fruit ilarviruses.
T. HO (1), I. E. Tzanetakis (1). (1) Department of Plant Pathology, Division of Agriculture, University of Arkansas, Fayetteville, AR, U.S.A.

Knowledge of the population structure of three berry-infecting ilarviruses, namely <i>Blackberry chlorotic ringspot virus</i> (BCRV), <i>Strawberry necrotic shock virus</i> (SNSV) and <i>Tobacco streak virus</i> (TSV), has grown considerably in the past few years. Sequence data, obtained in our laboratory or GenBank, were used for the development of TaqMan® quantitative PCR (qPCR) assays with absolute quantification. These assays were developed on the premise that they can detect all isolates of the three viruses studied to date. Total nucleic acids were extracted from virus-infected materials, digested with DNase and used for reserve transcription with specific and random primers, followed by qPCR. Standard curves were constructed and verified consistent detection of as low as 30 copies of the respective viruses. The assays, reproducible for both Ct values and calculated copy numbers, have been successfully tested on many berry fruit isolates.<p><p>Keywords: Virus-Viroid, Fruits-Nuts

View Presentation