Link to home

Cannot retrieve the URL specified in the Content Link property. For more assistance, contact your site administrator.

RNA interference-induced Heterodera glycines resistance in soybean.
J. LI (1), T. C. Todd (1), T. R. Oakley (1), H. N. Trick (1). (1) Kansas State University, Manhattan, KS, U.S.A.

The soybean cyst nematode (SCN), <i>Heterodera glycines</i>, is the most severe biotic stress limiting soybean production, accounting for $4 billion loss per year worldwide. Current SCN management approaches nematicides, crop rotation and resistant varieties all have serious limitations. To explore alternative methods of SCN control, we deployed an <i>in planta</i> transgenic approach for suppressing SCN populations by transferring RNA interference (RNAi) constructs containing inverted repeats of 25 different <i>Heterodera glycines</i> genes into soybean plants, separately. Transgenic roots were evaluated by PCR, RT-PCR, Southern blot, siRNA Northern blot and small RNA sequencing. <i>H. glycines</i> bioassay results performed on the composite plants indicated that 7 out of 25 RNAi constructs resulted in significant reduction for <i>H. glycines</i> eggs, while the other 18 RNAi constructs did not have significant effects. Composite plants expressing RNAi construct of <i>Y-025</i> and <i>R-001</i> gene showed consistent suppression of <i>H. glycines</i> HG type 2.7 and HG type 7 populations. Stacking two genes together in one construct did not lead to more significant reduction for <i>H. glycines</i> development. Our research results indicated that RNAi strategy may provide effective and durable SCN control.<p><p>Keywords: Nematode, Legumes, Beans

View Presentation