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The soybean cyst nematode (SCN), <i>Heterodera glycines</i>, is the most severe biotic stress limiting soybean production, accounting for $4 billion loss per year worldwide. Current SCN management approaches nematicides, crop rotation and resistant varieties all have serious limitations. To explore alternative methods of SCN control, we deployed an <i>in planta</i> transgenic approach for suppressing SCN populations by transferring RNA interference (RNAi) constructs containing inverted repeats of 25 different <i>Heterodera glycines</i> genes into soybean plants, separately. Transgenic roots were evaluated by PCR, RT-PCR, Southern blot, siRNA Northern blot and small RNA sequencing. <i>H. glycines</i> bioassay results performed on the composite plants indicated that 7 out of 25 RNAi constructs resulted in significant reduction for <i>H. glycines</i> eggs, while the other 18 RNAi constructs did not have significant effects. Composite plants expressing RNAi construct of <i>Y-025</i> and <i>R-001</i> gene showed consistent suppression of <i>H. glycines</i> HG type 2.7 and HG type 7 populations. Stacking two genes together in one construct did not lead to more significant reduction for <i>H. glycines</i> development. Our research results indicated that RNAi strategy may provide effective and durable SCN control.<p><p>Keywords: Nematode, Legumes, Beans