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Genome sequencing of Fusarium oxysporum f. sp. cubense tropical race 4 strain II5.
A. S. BERG (1), M. Dita (2), T. Nan (1), T. Shea (3), S. Zhou (4), W. Jonkers (5), Q. Zeng (3), S. Young (3), M. E. Beleza Yamagishi (6), P. Giachetto (6), R. Herai (7), M. Souza (8), C. Waalwijk (8), G. Haatje Jan Kema (8), H. Kistler (5), L. Ma (1). (1) University of Massachusetts at Amherst, Amherst, MA, U.S.A.; (2) Bioversity International, Turrialba, Costa Rica; (3) Broad Institute, Cambridge, MA, U.S.A.; (4) University of Wisconsin-Madison, Madison, WI, U.S.A.; (5) University of Minnesota, St. Pa

<i>Fusarium oxysporum</i> f. sp. <i>cubense</i> (<i>Foc</i>) is the causal agent of Fusarium wilt of banana, also known as Panama disease. Tropical race 4 (TR4) of <i>Foc</i> emerged in the early 1990s and is spreading throughout Asia and northern Australia. There are no effective treatments or viable export cultivars resistant to <i>Foc</i> TR4. A genome assembly of <i>Foc</i> TR4 strain II5 has been generated using Illumina sequencing technology. A combination of paired-end reads from 180 bp fragments and 5kb insert libraries delivered a high quality assembly with a total of 46.5M assembled bases in 418 scaffolds of 1.13 N50 scaffold. Based on kmer estimation, the II5 genome size is approximately 51Mb, including about 4.3Mb lineage specific (LS) sequences that are highly repetitive, a characteristic described in other LS sequences within <i>F. oxysporum</i> f. sp. <i>lycopersici</i>. An initial genome annotation was conducted, incorporating RNA-seq data generated with 454 sequencing technology. We will report the preliminary analysis of the genome and the annotation results, with a focus on detection of potential virulence factors, such as effectors, to further elucidate the methods of pathogenesis of TR4. Additionally, we will validate the completeness of the current assembly by comparing independently generated transcripts. Further understanding of the profile of the effectors secreted between the four races of <i>Foc</i> may speed development of TR4 resistant banana cultivars as well as integrated management strategies.<p><p>Keywords: Fungus, Tropical-Subtropical Crops, Banana

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