Welcome to the Diagnostic Assay Validation Network (DAVN) – a new community for diagnostic researchers, regulators, and professionals across government, industry, and academia to develop and share standard diagnostic method development and validation within the plant science community.
The DAVN was created to provide a working space for plant scientists to easily access statistical tools, registries of methods and controls, promote validated methods, and connect with assay developers, testers, and end-users via an online community (coming soon).
Learn more about the need and the vision for DAVN.
Using RCA for Detecting and Studying Diseases: Examples for Plant DNA Viruses
Broadcast Date: Wednesday, December 6 | 12:00pm - 1:00pm CST
This session will introduce the protocol for using RCA to amplify circular DNA, focusing on plant DNA viruses as case studies. We'll begin with a basic DNA extraction protocol and proceed to the amplification step using phi 29 DNA polymerase. The resulting DNA will then be ready for various applications, including sequencing, cloning, and diversity analysis.
Methods and Tools for Plant Pathogen Immunoassay Design, Development, and Analysis
Broadcast Date: Tuesday, February 6 | 12:00pm - 1:00pm CST
This session will review classical techniques and describe modern techniques for designing, generating, and applying immunoreagents in diagnostic assays, and analyzing data outputs from assays. At the end of this session, attendees will understand the biology and biochemistry behind immunoassays, terminology describing antibodies used in assays and various types of diagnostic immunoassays; novel techniques for discovery and design of antibody targets; and various methods for generating natural and synthetic antibodies. Attendees will learn where to access online APS open-source statistical tools and how to apply them to data outputs from immunoassays in plant pathogen diagnostics.
Isothermal Diagnostics for Critical Plant Pathogens: Design, Development, and Implementation
Broadcast date: Thursday, March 14 | 1:00pm - 2:00pm CST
Gain a comprehensive understanding of the fundamental principles of isothermal amplification techniques, explore real-world case studies that showcase successful applications of isothermal diagnostics in plant pathology, and understand how these techniques have been used to identify and manage plant diseases in diverse agricultural contexts.
Resources & News
Focus Issue: APS has published a DAVN focus issue. Find the DAVN focus issue here.
Webinar: Dr. Carrie Lapaire Harmon (University of Florida) led a DAVN webinar with the National Plant Diagnostic Network on March 1, 2023. You can access the webinar recording here.
Survey Results: Findings from the Specimen Management Plan Community Survey that was conducted by the Biodiversity Collections Network (BCoN) and the U.S. Culture Collection Network (USCCN) in partnership with the American Institute of Biological Sciences and the Natural Science Collections Alliance (published April 11, 2023)
Follow us on Twitter: catch our latest updates when you follow us @the_davn
Components of the dashboard coming in 2024:
Methods library: designed to help users find appropriate methods more quickly and explain their performance limitations to clientele.
Registry of expertise, testers, and end-users: supports development and validation of new assays through ring-testing and continuous improvement through end-use feedback
Registry of specimen and insilico cultures and collections and data: supports the development of validated protocols and use of appropriate positive and negative controls in testing laboratories
You can submit your Diagnostic Assay Validation related papers to any APS journal. Publications presenting new or adapted diagnostic tests or assays should clearly state the purpose for which the assay is developed, e.g., surveillance both pre- and post- detection, presumptive identifications, confirmatory diagnostics, regulatory clearance of plant products including seed, germplasm or plants, research, etc.
- Research manuscripts should give focus to assay validation parameters and explicitly state the purpose of the assay, assay fitness for the purpose, and which of the following validation parameters were addressed and how:
analytical specificity, diagnostic specificity, analytical sensitivity, diagnostic sensitivity, repeatability, and reproducibility. Validation parameters should be discussed in the body of the manuscript and reviewed in the discussion section. The validation parameters listed above should be explicitly addressed including those that were not addressed and why. Click here for more instructions on how to submit a manuscript.
View more instructions on how to submit a manuscript.
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Questions? Contact us at DAVN@scisoc.org