September
2007
, Volume
91
, Number
9
Pages
1,194
-
1,197
Authors
V.
Pahalawatta
,
Department of Plant Pathology, Washington State University, P.O. Box 99164-6430, Pullman, 99164
;
R.
Miglino
,
Dutch Flower Bulb Inspection Service, P.O. Box 300, 2160 AH Lisse, the Netherlands
;
K. B.
Druffel
,
Department of Plant Pathology, Washington State University, P.O. Box 99164-6430, Pullman, 99164
;
A.
Jodlowska
and
A. R.
van Schadewijk
,
Dutch Flower Bulb Inspection Service, P.O. Box 300, 2160 AH Lisse, the Netherlands
; and
H. R.
Pappu
,
Department of Plant Pathology, Washington State University, P.O. Box 99164-6430, Pullman, 99164
Affiliations
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RelatedArticle
Accepted for publication 26 April 2007.
Abstract
ABSTRACT
Dahlia mosaic, caused by Dahlia mosaic virus (DMV), is one of the most important viral diseases of dahlia. Molecular characterization of DMV showed the association of two distinct caulimoviruses (DMV-D10, DMV-Portland) and a D10-like sequence variant (DMV-Holland) with the disease. Using primers specific to these two viruses and the sequence variant, a polymerase chain reaction--based assay was used to determine their relative incidence in several dahlia samples from the United States and the Netherlands. Testing was done on samples collected in 2005 and 2006 in the United States and in 2006 in the Netherlands. Results indicated the predominance of DMV-D10 over DMV-Portland and DMV-Holland in both the United States and the Netherlands. Using conserved regions of the viral genome, primers were designed and used to detect all three sequences. Results suggested that DMV-D10 is predominantly associated with dahlia mosaic, but diagnostics should also include testing for DMV-Portland and DMV-Holland.
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© 2007 The American Phytopathological Society
