June
2006
, Volume
90
, Number
6
Pages
723
-
728
Authors
C.
Vaïanopoulos
,
A.
Legrève
,
C.
Lorca
, and
V.
Moreau
,
Unité de Phytopathologie, Université catholique de Louvain (UCL), Louvain-la-Neuve 1348, Belgium
;
S.
Steyer
,
Département Lutte biologique et Ressources phytogénétiques, Centre wallon de recherches agronomiques, CRA-W, Gembloux 5030, Belgium
; and
H.
Maraite
and
C.
Bragard
,
Unité de Phytopathologie, UCL, Belgium
Affiliations
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RelatedArticle
Accepted for publication 2 January 2006.
Abstract
ABSTRACT
In order to assess the occurrence of Wheat spindle streak mosaic virus (WSSMV) in Belgium, a reverse-transcription polymerase chain reaction (RT-PCR) was developed, targeting WSSMV isolates from Canada, France, Germany, Italy, and the United States. The primers also were designed for virus quantification by real-time RT-PCR with SYBR-Green. No cross-reaction with soilborne cereal viruses such as Barley mild mosaic virus, Barley yellow mosaic virus, Soilborne cereal mosaic virus, and Soil-borne wheat mosaic virus was observed. The RT-PCR and real-time quantitative RT-PCR allowed a more sensitive detection of WSSMV than enzymelinked immunosorbent assay. The incidence of WSSMV in Belgium was evaluated using a bioassay with wheat cvs. Cezanne and Savannah and rye cv. Halo, grown in 104 Belgian soils. The presence of WSSMV was detected from plants grown in 32% of the soils. The RT-PCR methods developed here, combined with large sampling, allowed WSSMV to be detected for the first time in Belgium. The real-time quantitative RT-PCR was developed as a tool for evaluating the resistance to WSSMV by quantifying the virus concentration in wheat cultivars.
JnArticleKeywords
Additional keywords:
Polymyxa graminis,
soilborne viruses
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ArticleCopyright
© 2006 The American Phytopathological Society