December
1999
, Volume
83
, Number
12
Pages
1,101
-
1,104
Authors
H. M.
Griffiths
and
W. A.
Sinclair
,
Department of Plant Pathology, Cornell University, Ithaca, NY 14853-4203
;
E.
Boudon-Padieu
and
X.
Daire
,
Institut National de Recherche Agronomique, Station de Recherches sur les Phytoplasmes, BV 1540, 21034 Dijon Cedex, France
;
I.-M.
Lee
,
USDA-ARS, Molecular Plant Pathology Laboratory, Beltsville, MD 20705
;
A.
Sfalanga
,
Istituto di Patologia e Zoologia Forestale ed Agraria, University of Firenze,
50100 Firenze, Italy
; and
A.
Bertaccini
,
Istituto di Patologia Vegetale, Universita degli Studi di Bologna, 40126
Bologna, Italy
Affiliations
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RelatedArticle
Accepted for publication 25 August 1999.
Abstract
ABSTRACT
Restriction fragment length polymorphism (RFLP) analyses were performed on polymerase chain reaction (PCR) amplimers of phytoplasmal DNA from eight samples obtained from Ulmus spp. (elms) affected by elm yellows (EY) in Italy and the United States, from Catharanthus roseus infected with strain EY1, and from five other plant species infected with phytoplasmas of the EY group sensu lato (group 16SrV). RFLP profiles obtained with restriction enzyme TaqI from ribosomal DNA amplified with primer pair P1/P7 differentiated elm-associated phytoplasmas from strains originally detected in Apocynum cannabinum, Prunus spp., Rubus fruticosus, Vitis vinifera, and Ziziphus jujuba. RFLP profiles obtained similarly with BfaI differentiated strains from A. cannabinum and V. vinifera from other phytoplasmas of group 16SrV. Elm-associated strains from within the United States had two RFLP patterns in ribosomal DNA based on presence or absence of an RsaI site in the 16S--23S spacer. Elm-associated phytoplasma strains from Italy were distinguished from those of American origin by RFLPs obtained with MseI in the same fragment of non-ribosomal DNA. Strain HD1, which was discovered in A. cannabinum associated with EY-diseased elms in New York State, was unique among the strains studied.
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© 1999 The American Phytopathological Society