March
2004
, Volume
17
, Number
3
Pages
292
-
303
Authors
Anke
Becker
,
1
Hélène
Bergès
,
4
Elizaveta
Krol
,
1
,
2
Claude
Bruand
,
4
Silvia
Rüberg
,
2
Delphine
Capela
,
4
Emmanuelle
Lauber
,
4
Eliane
Meilhoc
,
4
Frédéric
Ampe
,
4
Frans J.
de Bruijn
,
4
Joëlle
Fourment
,
4
Anne
Francez-Charlot
,
4
Daniel
Kahn
,
4
Helge
Küster
,
2
,
3
Carine
Liebe
,
4
Alfred
Pühler
,
2
Stefan
Weidner
,
2
and
Jacques
Batut
4
Affiliations
1Institut für Genomforschung, Centrum für Biotechnologie, Universität Bielefeld, Postfach 100131, D-33501 Bielefeld, Germany; 2Lehrstuhl für Genetik, Universität Bielefeld, Postfach 100131, D-33501 Bielefeld, Germany; 3International NRW Graduate School in Bioinformatics and Genome Research, Universität Bielefeld, Postfach 100131, D-33501 Bielefeld, Germany; 4Laboratoire des Interactions Plantes-Microorganismes, UMR INRA 441-CNRS 2594, BP27-31326 Castanet-Tolosan cedex, France
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RelatedArticle
Accepted 7 October 2003.
Abstract
Sinorhizobium meliloti is an α-proteobacterium that alternates between a free-living phase in bulk soil or in the rhizosphere of plants and a symbiotic phase within the host plant cells, where the bacteria ultimately differentiate into nitrogen-fixing organelle-like cells, called bacteroids. As a step toward understanding the physiology of S. meliloti in its free-living and symbiotic forms and the transition between the two, gene expression profiles were determined under two sets of biological conditions: growth under oxic versus microoxic conditions, and in free-living versus symbiotic state. Data acquisition was based on both macro- and microarrays. Transcriptome profiles highlighted a profound modification of gene expression during bacteroid differentiation, with 16% of genes being altered. The data are consistent with an overall slow down of bacteroid metabolism during adaptation to symbiotic life and acquisition of nitrogen fixation capability. A large number of genes of unknown function, including potential regulators, that may play a role in symbiosis were identified. Transcriptome profiling in response to oxygen limitation indicated that up to 5% of the genes were oxygen regulated. However, the microoxic and bacteroid transcriptomes only partially overlap, implying that oxygen contributes to a limited extent to the control of symbiotic gene expression.
JnArticleKeywords
Additional keywords:
macroarray,
root nodule.
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© 2004 The American Phytopathological Society