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Transcript Profiling in the Barley Mildew Pathogen Blumeria graminis by Serial Analysis of Gene Expression (SAGE)

¹Department of Physiology, Carlsberg Laboratory, Gamle Carlsberg Vej 10, Copenhagen Valby, DK-2500, Denmark; ²Department of Yeast Genetics, Carlsberg Laboratory, Gamle Carlsberg Vej 10, Copenhagen Valby, DK-2500, Denmark; ³Australian Centre for Necrotrophic Fungal Pathogens, Division of Science and Engineering, Murdoch University, WA 6150, Australia

The fungal pathogen Blumeria graminis f. sp. hordei develops on the barley leaf via distinct, morphologically well-defined stages. After landing on a host plant, the conidia rapidly germinate to form a primary germ tube. Subsequently, an appressorial germ tube emerges from the conidium and differentiates an appressorium from which penetration of the host cell wall is attempted. We have used serial analysis of gene expression to provide a measurement of messenger RNA contents in ungerminated conidia, during conidial germination, and during appressorium formation. The resulting data provide a resource for the characterization of changes in transcript accumulation during early development of B. graminis.