Previous View
 
APSnet Home
 
Plant Disease Home


VIEW ARTICLE

Research

Isolation of Cherry Leaf Roll and Brome Mosaic Viruses from European Beech and Transmission to Beech Seedlings. JOACHIM HAMACHER, Associate Professor, Institute fur Pflanzenkrankheiten, Rheinische Friedrich-Wilhelms-Universitat, Nussallee 9, 53115 Bonn, Germany. ANDREA QUADT, Research Associate, Institut fur Pflanzenkrankheiten, Rheinische Friedrich-Wilhelms-Universitat, Nussallee 9, 53115 Bonn, Germany. Plant Dis. 78:849-853. Accepted for publication 16 May 1994. Copyright 1994 The American Phytopathological Society. DOI: 10.1094/PD-78-0849.

Cherry leaf roll virus (CLRV) and brome mosaic virus (BMV) were transmitted from foliage of European beech to herbaceous plants and then back-transmitted to beech seedlings. Beech sampling sites were located in a nature reserve in Northrhine-Westfalia for CLRV and near the village of Hilberath, between the borders of the states of Rhineland-Pfalz and Northrhine-Westfalia, for BMV. Viruses were identified by bioassay, enzyme-linked immunosorbent assay, and immune electron microscopy. Transmission succeeded when seedlings in the four-leaf stage were inoculated by stem slashing. Infection rates averaged 24% for BMV and 17% for CLRV. Injection of concentrated BMV suspension into stratified beechnuts resulted in 15% infected seedlings 1 yr after infection. Symptoms developed in some inoculated beech seedlings about 4 wk after inoculation. Electron microscopy of infected tissue showed ultrastructural alterations.