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First Report of Tomato Spotted Wilt Virus in Greenhouse Crops in Oklahoma. J. L. Sherwood, Department of Plant Pathology, Oklahoma State University, Stillwater 74078. S. L. von Broembsen, Department of Plant Pathology, Oklahoma State University, Stillwater 74078. Plant Dis. 75:750. Accepted for publication 20 February 1991. Copyright 1991 The American Phytopathological Society. DOI: 10.1094/PD-75-0750D.

Tomato spotted wilt virus (TSWV) was found in ornamentals (impatiens, New Guinea impatiens, wax begonia, dahlia, ivy geranium, lemon geranium, browallia) and tomato from greenhouses in the counties in Oklahoma of Cherokee, Oklahoma, and Cleveland in 1990. TSWV was identified by enzyme-linked immunosorbent assay (ELISA) using monoclonal antibody to the kttuce strain (L) (2) and polyclonal antibody to the impatiens strain (1) (1) provided by J. W. Moyer of North Carolina State University, Raleigh. Symptomatic samples generally were positive in ELISA for only one strain but occasionally were positive for both strains. This was supported by inoculation to Petunia X hybrida Hort. Vilm.-Andr., Nicotiana benthamiana Domin, and Datura stramonium L. Both TSWV-L and TSWV-I produce necrotic localized lesions on P. X hybrida. D. stramonium is a systemic host for TSWV-L, and N. benthamiana is readily infected by TSWVI. Although TSWV-L has been found in Oklahoma in peanut, this is the first report of TSWV-I in Oklahoma and the first report of TSWV-L in horticultural crops in Oklahoma.

References: (1) M. Law and J. Moyer. J. Gen. Virol. 71:933, 1990. (2) J. L. Sherwood et aI. Phytopathology 79:61, 1989.