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Disease Note.

Glomerella cingulata Associated with Leaf and Shoot Blight of Hybrid Poplar. G. Newcombe, Washington State University, Puyallup 98371-4998. J. M. Staley, and G. A Chastagner. Washington State University, Puyallup 98371-4998. Plant Dis. 75:1186. Accepted for publication 6 August 1991. Copyright 1991 The American Phytopathological Society. DOI: 10.1094/PD-75-1186C.

Leaf samples collected throughout Taiwan from hot and sweet pepper (Capsicum annuum L.) showing symptoms typica.1 of vir~s infection were inoculated to Nicotiana glutinosa L. on whICh all SIX tobamoviruses so far reported to infect pepper are known to cause )ocallesions. After four single local lesion transfers, the virus isolates were maintained in N. tabacum L. 'Samsun'; particles typically shaped like tobamoviruses were detected by electron microscopy. Twenty isolates were further characterized by inoculation of a set of differential hosts (2), including C. frutescens L., Eryngium planum L., Lycopersicon esculentum Mill., N. sylvestris Speg., and Petunia X hybrida Hort. Vilm.-Andr. Seventeen isolates were characterized as tomato mosaic virus (ToMV) and one each as tobacco mosaic virus (TMV), pepper mild mottle virus (PMMV), and tobacco mild green mosaic virus (TMGMV). Virus identity was further confirmed by agar gel double-diffusion tests, using five tobamovirus antisera provided by C. Wetter. Distinct precipitin lines without spurs were formed only with homologous antisera. The ToMV isolates were further straintyped on the L. esculentum differentials CSTMW-18, Perou, and Delissa. Four of the 17 ToMV isolates were strain 1 and 13 were strain O. Additional inoculation of a set of five Capsicum species (1) showed that the PMMV isolate corresponded to pathotype P1.2, and the TMGMV isolate to pathotype P0, of the so-called pepper strain ofTMV. To our knowledge, this is the first isolation and identification of PMMV and TMGMV in Taiwan.

References: (1) A. Th. B. Rast. Capsicum Newsl. 7:20, 1988. (2) C. Wetter et al. J. Phytopathol. 119:333, 1987.