First Report of Southern Bean Mosaic Virus in Nicaragua. A. L. Fuentes, Department of Plant Science, University of British Columbia, Vancouver, B.C.. P. K. Anderson, Harvard School of Public Health, Department of Population Sciences, 665 Huntington Ave., Boston, MA 02115. Plant Dis. 74:938. Accepted for publication 5 July 1990. Copyright 1990 The American Phytopathological Society. DOI: 10.1094/PD-74-0938B.
Bean (Phaseolus vulgaris L.) is the most important staple protein
in the Nicaraguan diet. Although eight viruses, including southern
bean mosaic virus (SBMV), have been putatively reported to infect
bean in Nicaragua, only bean common mosaic has been definitively
identified (1). Leaves of bean plants showing light chlorotic mottling
and slight crumpling were collected from bean fields in the Sebaco
Valley of Nicaragua during November 1988. The causal agent was
identified as the bean strain of SBMV on the basis of local lesions
produced on P. vulgaris cv. Pinto, immunosorbent electron microscopy
of leaf dips, serological reactions in Ouchterlony double-diffusion tests
and double-antibody sandwich enzyme-linked immunosorbent assay.
Antiserum to SBMV-B and SBMV-C to produce antiserum were
obtained from the sera and virus collection of the Vancouver
Agriculture Canada Research Station. The virus did not infect cowpea
(Vigna unguiculata L.).