Tomato Spotted Wilt Virus in Peanut in Alabama. A. K. Hagan, Department of Plant Pathology and Entomology, Auburn University, Auburn, AL 36849. J. R. Weeks, J. C. French, R. T. Gudauskas, J. M. Mullen, W. S. Gazaway, and R. Shelby. Departments of Plant Pathology and Entomology, Auburn University, Auburn, AL 36849. Plant Dis. 74:615. Accepted for publication 15 March 1990. Copyright 1990 The American Phytopathological Society. DOI: 10.1094/PD-74-0615B.
Tomato spotted wilt virus (TSWV) was identified in peanut (Arachis
hypogaea L.) in Alabama in the summer of 1986. Virus identification
was based on enzyme-linked immunosorbent assay (ELISA; lettuce
strain, Agdia Inc., Mishawaka, IN) of leaf sap from symptomatic
plants and reactions on Nicotiana benthamiana Domin and N. tabacum
L. 'Burley 21' rub-inoculated with sap. In addition to typical TSWV
symptoms (1), chlorosis of foliage, limb collapse, and premature plant
death were associated with TSWV infections. Surveys (1986-1989),
with confirmatory ELISA tests, showed presence of TSWV in all of
Alabama's peanut-producing counties. TSWV-infected plants were
found in 82% of the 288 fields checked over the survey period; highest
incidence within fields (3.1%) and statewide (0.49%) was seen in 1986.