Survey of Spring Oats for Barley Yellow Dwarf Viruses in Illinois. O. I. Azzam, Department of Plant Pathology, University of Illinois, Urbana 61801. C. J. D’Arcy, Department of Plant Pathology, University of Illinois, Urbana 61801. Plant Dis. 73:610. Accepted for publication 21 March 1989. Copyright 1989 The American Phytopathological Society. DOI: 10.1094/PD-73-0610D.
During the spring of 1987, 1,215 samples of spring oats (Avena
sativa L.) were collected in Madison, Champaign, Woodford, Warren,
and DeKalb counties, Illinois. At each site on each of three sampling
dates, 45 samples were collected (regardless of symptoms) in a W
pattern in I ha and tested for the PAY, MAV, RPV, and RMV
serotypes of barley yellow dwarf virus (BYDV) by direct doubleantibody
sandwich enzyme-linked immunosorbent assay (ELISA).
RMV was not detected at any location. PAY and RPV were detected
at all locations, as early as 17 April in Champaign County. The
incidences of P A V and RPV from all plants sampled ranged from
2 to 64% and from 2 to 88%, respectively. Highest incidences of both
strains were in May samples [rom Woodford County. MAV was
detected in lower incidences (2-16%) only in samples from the central
region of the state (Champaign, Woodford, and Warren counties).
The presence of MA V serotypes was confirmed in triple-antibody
sandwich ELISA with the MA V -specific MAFF2 monoclonal
antibody from L. Torrance. In the last previous survey for BYDV
in Illinois during 1967-1968 (1), about 75% of the isolates were PAY
and about 20% were RPV; single isolates of RMV and MAV were
found. Twenty years later, 55% were PAY, 39% were RPV, and 6%