**A Quantitative Method for Estimation of Teliospores of ***Tilletia indica* in Soil. L. E. Datnoff, Research Affiliate, Foreign Disease-Weed Science Research Unit, USDA-ARS, Frederick, MD 21701. M. H. Royer, M. R. Bonde, and J. M. Prescott. Research Plant Pathologists, Foreign Disease-Weed Science Research Unit, USDA-ARS, Frederick, MD 21701, and Seed Health Pathologist, International Center for the Improvement of Maize and Wheat, Lisbon 27, Apdo. Postal 6-641, Mexico 06600 DF. Plant Dis. 72:209-212. Accepted for publication 8 September 1987. This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 1988. DOI: 10.1094/PD-72-0209.

A bubbling-flotation-sieving method utilizing 50% glycerol was developed for the extraction of teliospores of *Tilletia indica* from artificially and naturally infested soil. A standard curve was prepared to estimate the number of teliospores recovered from soil artificially infested with about 50–5,000 teliospores per 10 g of soil. A log_{10} transformation stabilized the variances of the number of teliospores recovered relative to the number actually present in soil. A prediction equation then was developed to estimate the number of teliospores in a naturally infested Mexican soil (*X*) based on the number of teliospores recovered (*Y*): log_{10} *X* = (log_{10} *Y* + 2)/1.47. The mean number of teliospores in Mexican soil was estimated to be between 476 and 1,617 per 10 g of soil. The sample sizes required for margins of error within 5, 10, 15, and 20% of the mean number of teliospores recovered also were determined. This methodology for the extraction and enumeration of teliospores of *T. indica* from Mexican clay soil will be useful in future studies on the ecology and epidemiology of the fungus.